H7520-02
HS6ST3 (Heparan sulfate 6-O-sulfotransferase 3)
Size
£863.00
- SKU:
- H7520-02
- Application:
- IHC-Fr
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant corresponding to aa28-470 from mouse HS6ST3 expressed in CHO cells (NP_056635).
- Format:
- Supplied as a lyophilized powder from PBS, pH7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- HS6ST3 (Heparin sulfate 6-O-sulfotransferase 3) is a 60-62kD member of the sulfotransferase 6 family of enzymes. It is one of three 6-O sulfotransferase isoforms, all of which essentially perform the same function, but all of which are the products of distinct genes. HS6ST3 is known to sulfate the #6 position of glucosamine sulfate that is linked to a 2-O-sulfated iduronic acid in heparin sulfate. The molecule shows a temporal expression pattern, being found in postnatal cerebellar Purkinje cells and cerebral cortical pyramidal cells in layers II/III and V. Mouse HS6ST3 is a 470aa type II transmembrane glycoprotein. It contains a short four aa cytoplasmic N-terminus coupled to an extended 443aa lumenal domain (aa28-470). The lumenal domain contains a stem region (aa28-83) that mediates oligomerization and Golgi localization, plus a sulfotransferase enzyme domain (aa142-409). Cleavage within the stem region by b-secretase generates a soluble form of HS6ST3. Over aa28-470, mouse HS6ST3 shares 99-95aa identity with rat and human HS6ST3, respectively. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice