H7446-50B-HRP
Hrk (Activator of Apoptosis Harakiri, BH3-interacting Domain Containing Protein 3, BID3, DP5, Harakiri BCL2 Interacting Protein (Contains Only BH3 Domain), Neuronal Death Protein DP5) (HRP)
Size
£882.00
- SKU:
- H7446-50B-HRP
- Application:
- WB
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 15aa near the center of human HRK (NP_003797).
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and hematopoietic cell apoptosis, in particular to growth factor deprivation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: Detects a band at ~13kD in mouse pancreas tissue lysate. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice