H6098-72R

HLA Class 1 Antigen G (HLA Cass I Histocompatibility Antigen alpha Chain G, HLA G Antigen, HLA G, HLAG, HLA-G, HLA-6.0, Major Histocompatibility Complex Class I G, MHC-G)

Size

£621.00

SKU:: H6098-72R
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human HLA-G refolded with beta2-microglobulin and peptide.
Clone:: MEM-G/9
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45kD and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. Applications: Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: frozen Flow Cytometry: 0.3-4ug/ml; positive control: JEG-3 human choriocarcinoma cell line. Immunocytochemistry: 2-5ug/ml ELISA: capture antibody in a sandwich ELISA for analysis of human HLA-G in combination 171665 or 366956. Coating antibody 10ug/ml, detection antibody (biotin or peroxidase conjugate) 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice