H6098-68
HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E, MHC Class I Antigen E)
Size
£512.00
- SKU:
- H6098-68
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human HLA-E denaturated heavy chain.
- Clone:
- MEM-E/02
- Format:
- Supplied as a liquid in PBS, pH7.4, 15mM sodium azide.
- Extra Details:
- HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin sections): 5-10ug/ml. Heat antigen retrieval (sodium citrate); incubate 1hour at RT; detection DAB Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Human placenta Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice