H6098-62A
HLA Class 1 Antigen Bw6 (FITC)
Size
£1,823.00
- SKU:
- H6098-62A
- Purity:
- ≥98%, Purified by Ion Exchange chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Clone:
- 0.L.5
- Format:
- Supplied as a liquid in PBS, 1% BSA., 0.09% sodium azide Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Applications: Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, and Chimerism Studies. Other applications have not been tested. Recommended Dilution: Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution. Optimal dilutions to be determined by the researcher. Cytotoxicity: Expected Results: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at 4°C. Aliquots are stable at 4°C for 12 months after receipt. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice