H6098-52D
HLA Class 1 Antigen B27 (FITC)
Size
£960.00
- SKU:
- H6098-52D
- Application:
- Flow Cytometry
- Purity:
- Purified by Ion Exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
- Clone:
- 3H2219 (HLA-ABC-m3)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- This is a cytotoxic antibody which detects HLA-B27 alloantigen. This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27+ and precipitates cell surface molecules of 43 and 12kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Neat; Use 10ul to label 10e6 cells or 100ul of whole blood. Optimal working dilutions to be determined by researcher. Recommended Negative Control: I1904-78R3: IgG1 Murine Negative Control (FITC) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice