H6098-49

HLA Class 1 Antigen B14

Size

£1,662.00

SKU:: H6098-49
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: 4i120
Format:: Supplied as a lyophilized powder. Reconstitute to 100ul with sterile dH2O to make a 10X stock solution.
Extra Details:: Applications: Suitable for use in Cell Typing, Tissue Staining and Chimerism Studies. Other applications not tested. Recommended Dilutions: Cytotoxicity: 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution. Optimal dilutions to be determined by the researcher. Expected Results: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results. Storage and Stability Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.