H6098-39F
HLA Class 1 Antigen ABC
Size
£691.00
- SKU:
- H6098-39F
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Membrane of human tonsil cells.
- Clone:
- 3H2211 (W6/32)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- Human Leukocyte Antigen (HLA) class I which is also known as human class I Major Histocompatibility Complex (MHC). HLA class I is encoded by three HLA alleles: HLA-A, HLA-B and HLA-C. HLA class I is a protein dimer composed of an alpha chain (Mr =44kD) and a beta chain which is also known as b2 microglobulin (Mr =12kD). The smaller beta chain bears homology with immunoglobulin molecules. The alpha chain contains four distinct regions: a cytoplasmic region located at the carboxyl terminus of the protein, followed by a transmembrane region, an immunoglobulin-like region and a peptide-binding region located at the amino terminus. This peptide binding region contains a cleft which can bind peptides consisting of 9-11 amino acid residues. HLA class 1 specifically targets the bound peptide to T cells while interacting with the T-cell receptor. HLA class 1 appears to preferentially target CD8+ T cells, whereas HLA class II preferentially targets CD4+ T cells. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells of 100ul whole blood or 1x10e6 cells in suspension Immunohistochemistry: Frozen Optimal working dilutions to be determined by researcher. Recommended Positive Control: Tonsil Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice