H6007-10A

HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (HRP)

Size

£761.00

SKU:: H6007-10A
Purity:: Purified by ion affinity and reverse phase HPLC to ≥ 99% as determined by SDS-PAGE and HPLC.
Storage Conditions:: 4°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 10mM Tris, pH 8.0, 2.5% BSA, 0.01% thimerosal. Labeled with HRP.
Extra Details:: Peroxidase conjugated tat HIV-1 (E.coli). Molar Ratio: 2-4 peroxidase molecules per molecule of tat. Specificity: Peroxidase conjugated tat binds to murine monoclonal antibodies of defined epitope specificity and rabbit polyclonal antibodies in ELISA and Western ELISA. Applications: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1:500 range depending on the concentration of antibody in the test system. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.