H6004-18B

HIV-1 nef, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)

Size

£787.00

SKU:: H6004-18B
Purity:: Purified by glutathione affinity chromatography and the purified protein is thrombin-cleaved to produce nef of ≥95% purity, as determined by SDS-PAGE, reduced.
Storage Conditions:: 4°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: Coupling Ratio: ~5-10 moles of biotin per mole nef. Biological Activity: Binds to solid phase murine anti-nef monoclonal antibodies as determined by capture ELISA using strepavidin-HRP as a secondary reagent. Applications: Suitable for use in T-Cell Research, ELISA and Western ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 Capture ELISA: 1-10ng Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.