HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1) (HRP)

Size

£639.00

SKU:: H6003-34H1
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Format:: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% thimerosal. Labeled with horseradish peroxidase (HRP).
Extra Details:: Molar Ratio: 2-4 peroxidase molecules per molecule of antibody. Biological Activity: Peroxidase conjugated Rabbit anti-gp120 antibody binds to gp120 in CD4/gp120 capture ELISA and Western ELISA. Applications: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1:1000 depending on the concentration of gp120 in the test system. CD4/gp120 capture ELISA may be performed in 1 : 500 dilution range depending on the capture ligand CD4 concentration and gp120 concentration in the test sample. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.