H6003-34F3

HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1) (FITC)

Size

£614.00

SKU:: H6003-34F3
Purity:: ≥95% Purified by gel exclusion chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: HIV-1 gp120.
Clone:: 5i20
Format:: Supplied as a liquid in 10mM Tris, pH 8.0. No stabilizers or preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: HIV is a member of the genus Lentivirus, part of the family of Retroviridae, which use reverse transcriptase to convert the viral RNA genome into double stranded DNA. This is then integrated into the cell’s DNA, which is followed by latency or active HIV replication. HIV primarily infects CD4+ T lymphocytes, resulting in their dramatic decline. When CD4+ T cell numbers decline below a critical level, cell-mediated immunity is lost, and the body becomes progressively more susceptible to opportunistic infections. If untreated, this can lead to AIDS (Acquired Immunodeficiency Syndrome). Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The surface subunit, gp120, initiates the entry process by interacting sequentially with the CD4 receptor and a co-receptor CCR5 or CXCR4, thereby inducing a conformational change that allows the transmembrane (TM) gp41 subunit to mediate fusion between viral and target cell membranes. Cleavage of Env into its gp120 and gp41 components is necessary for activation of its fusogenic activity. Applications: Suitable for use in Dot Blot, FACS, Immunohistochemistry and Direct Immunofluorescence. Other applications not tested. Recommended Dilutions: Direct Immunofluorescence: 1:50 Dot Blot: 1:200 Optimal dilutions to be determined by the researcher. F/P Ratio: 3-4 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice