H6003-34F1
HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1) (HRP)
Size
£665.00
- SKU:
- H6003-34F1
- Purity:
- ≥95%
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HIV-1 gp120
- Clone:
- 5i20
- Format:
- Supplied as a liquid in 10mM Tris, pH 8.0, 1% BSA, 0.01% thimerosal. Labeled with horseradish peroxidase (HRP)
- Extra Details:
- Molar Ratio: 2-4 peroxidase molecules per molecule of mAb. Biological Activity: Peroxidase conjugated Murine anti-gp120 mAb binds to gp120 in CD4/gp120 capture ELISA and Western ELISA. Applications: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1 : 5000 depending on the concentration of gp120 in the test system. CD4/gp120 capture ELISA may be performed in 1 : 500 dilution range depending on the capture ligand CD4 concentration and gp120 concentration in the test sample. Typical assays with 100ng of solid phase CD4 will detect at least 10ng/ml gp120. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice