H6003-25M

HIV-1 p24, Core (Human Immunodeficiency Virus-1)

Size

£646.00

SKU:: H6003-25M
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to HIV-1 P24
Clone:: 9A96
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
Extra Details:: Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Structural studies demonstrated two distinct domains (N and C-terminal domains) that have different roles in the virus morphogenesis. Additionally, the level of p24 found in the blood serum is used as an indicator of HIV progression. HIV-1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Capture): 4ug/ml. Coat 100ul/well with TMB substrate. Western Blot: 0.1ug/ml antibody with 0.1ug /well of Recombinant HIV P24. Optimal dilutions to be determined by the researcher. Matched Pair: Capture: H6003-25M Dectection: H6003-01T Hybridoma: Sp2/0-AG14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice