H6003-03C

HIV-1 env gp41, Strain ADA (M Tropic), Recombinant (Human Immunodeficiency Virus-1)

Size

£887.00

SKU:: H6003-03C
Purity:: Purified by affinity chromatography and by preparative electrophoresis to ≥ 99% purity as determined by SDS-PAGE, reduced.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 10mM Tris, pH 8.0, SDS (< 0.01%). No stabilizers or preservatives added.
Extra Details:: Truncated Recombinant HIV-1 ADA M-Tropic envelope protein gp41 produced as a fusion protein in an E. coli Expression System. Truncated gp41 protein mol.wt: 25kD. Specificity: This protein binds to HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA. Applications: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 0.1-0.5mg/ml. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.