H6003-03B

HIV-1 env gp41, Strain YU2 (M Tropic), Recombinant (Human Immunodeficiency Virus-1)

Size

£884.00

SKU:: H6003-03B
Purity:: ≥99% (SDS-PAGE, reduced) Purified by affinity chromatography.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 10mM Tris, pH 8.0, <0.01% SDS. No stabilizers or preservatives added.
Extra Details:: Truncated Recombinant HIV-1 YU2 M-Tropic envelope protein gp41 produced as a fusion protein in an E. coli Expression System. Truncated gp41 protein mol.wt: 25kD. Specificity: H6003-03B binds to HIV-1 converted human serum polyclonal antibodies in ELISA and Western Blot. Applications: Suitable for use in immunization and T-cell activation. Other applications not tested. Recommended Dilutions: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.