H6003-01T
HIV-1, p24 (Human Immunodeficiency Virus-1) (HRP)
Size
£800.00
- SKU:
- H6003-01T
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified recombinant HIV-1 P24 (core protein).
- Clone:
- 10g187 (473)
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.0±0.1, 50% glycerol. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1000-2000 Western Blot: 0.1ug/ml Clear visible band with 0.1ug/well Recombinant HIV P24. Optimal dilutions to be determined by the researcher. Matched Pair: Capture: H6003-25M Dectection: H6003-01T Hybridoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice