H5305-FITC

Histones, pan (FITC)

Size

£1,170.00

SKU:: H5305-FITC
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified nuclear fractions of HeLa cells.
Clone:: 2Q2283
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail histones are involved with the structure of the nucleosomes of the 'beads on a string' structure. Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: Detects a band at ~20kD Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.