Histone H4, acetylated (Lys12) (Not for Export EU)

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£927.00

SKU:: H5110-17E
Purity:: Serum depleted of non-acetyl reactive antibodies.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Ovalbumin-conjugated, synthetic peptide containing the sequence (...LG[acK]GG...) in which acK corresponds to acetyl-lysine 12 of yeast Histone H4.
Format:: Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Extra Details:: Applications: Suitable for use in Peptide Inhibition, Peptide Dot Blot, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Peptide Inhibition: 10uM of a histone H4 peptide containing acetyl-lysine 12 abolished detection of histone H4 by anti-acetyl-Histone H4 (Lys12) in immunoblot analysis of HeLa acid extracts. Little to no signal reduction was observed with 10uM of histone H4 peptides containing acetyl-lysine 5, acetyl-lysine 8 or acetyl-lysine 16. Peptide Dot Blot: A 1:1,000 detects serial dilutions (25, 5, 1 and 0.2uM) of a histone H4 peptide containing acetyl-lysine 12 spotted onto PVDF, and not histone H4 peptides containing acetyl-lysine 5, acetyl-lysine 8 or acetyl-lysine 16. Western Blot: 1:3,000-1:10,000 detects acetyl-Histone H4 (Lys12) in acid extracted proteins from HeLa cells. The antibody did not detect unacetylated recombinant Histone H4. Immunoprecipitation: 2-6ul immunoprecipitated the Ime2 promoter from wild type yeast chromatin and not from mutant yeast chromatin containing a histone H4 K12R mutation. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice