H5110-14R1
Histone H3, dimethyl (Lys79)
Size
£1,004.00
- SKU:
- H5110-14R1
- Purity:
- Supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide ccorresponding to the sequence (...DFme2KTD...), where me2K corresponds to dimethyl-lysine at position 79 of histone H3 conjugated to KLH. The immunizing sequence is identical in human, mouse, rat and yeast.
- Clone:
- 5H66
- Format:
- Supplied as a liquid in 0.05% sodium azide.
- Extra Details:
- Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Applications: Suitable for use in Western Blot, Dot Blot and Histone-Peptide assay. Chromatin Immunoprecipitation (ChIP) has been reported by an independent laboratory. Other applications not tested. Recommended Dilution: Western Blot: 1:4000 Recombinant Histone H3 or acid extracted HeLa cell proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-14R1. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Peptide Dot Blot Analysis: 1:2500 dilution of this lot detected the immunizing peptide containing dimethyl-lysine 79. Some crossreactivity with peptide containing monomethyl-lysine 79 was also seen. No crossreactivity to other related histone peptides was found. The antibody did not recognize a histone peptide containing the dual modification of dimethyl-lysine 79 and phospho-threonine 80. Histone-Peptide Specificity Analysis: 1:25-1:1600 dilution of this lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. Peptide containing dimethyl-lysine 79 was readily detected. Some crossreactivity with peptide containing monomethyl-lysine 79 was seen. The antibody did not detect a histone H3 peptide containing the dual modification of dimethyl-lysine 79 and phosphothreonine 80. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice