H5110-14B
Histone H3, phosphorylated (Ser10) (Mitosis Marker)
Size
£1,023.00
- SKU:
- H5110-14B
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Linear peptide coresponding to human Histone H3 at Ser10
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml detected histone H3 on 10ug of Colemid treated HeLa acid extract. Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B. Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system. Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate. Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-47H: IgG, H&L (HRP) Pab GtxRt I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab GtxRt I1904-47T: IgG, H&L (HRP) Pab GtxRt I1904-50T1: IgG, H&L (HRP) Pab GtxRt I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab GtxRt I1904-40D: IgG, H&L (FITC) Pab Gt xRb I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb I1904-46N: IgG, H&L (FITC) Pab Gt xRb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after recei. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice