H5110-13K

Histone H3, phosphorylated (Ser10)

Size

£633.00

SKU:: H5110-13K
Purity:: Ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic phosphopeptide corresponding to residues around Ser10 of human histone H3.
Clone:: 3A50(6G3)
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Extra Details:: Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostain- ing with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase. Applications: Suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IF-IC): 1:100 Immunofluorescence (IF-F): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:25 Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% nonfat dry milk, 0.1% Tween-20 overnight at 4°C with gentle shaking. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice