Histone H3, acetylated (Lys23) (Not for Export EU)

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£927.00

SKU:: H5110-13A3
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated, synthetic peptide (KQLASAcKAARK-C) corresponding to aa18- 27 of yeast histone H3 acetylated on lysine 23, with a C-terminal cysteine added for conjugation purposes.
Format:: Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Extra Details:: Applications: Suitable for use in Western Blot and ChIP. Other applications not tested. Recommended Dilutions: Western Blot: 1:100,000-1:200,000 detected acetyl-histone H3 (Lys23) in acid extracts from sodium butyrate treated HeLa cells. The antibody does not detect unacetylated recombinant histone H3. Chromatin Immunoprecipitation (ChIP): 0.5ul preferentially immunoprecipitates chromatin from wild type yeast, compared to yeast containing a histone H3 K23R substitution. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.