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H5110-13A2

Histone H3, acetylated (Lys14) (Not for Export EU)

Size

£971.00

SKU:
H5110-13A2
Purity:
Serum
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Ovalbumin-conjugated, synthetic peptide (KSTGGAcKAPRK-C) corresponding to aa9-18 of yeast histone H3 acetylated on lysine 14, with a C-terminal cysteine added for conjugation purposes.
Format:
Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Extra Details:
Applications: Suitable for use in Western Blot, Beadlyte Histone-Peptide Specificity Assay and Chromatin IP. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 detected acetyl-Histone H3 (Lys14) in acid extracts from sodium butyrate treated HeLa cells Beadlyte Histone-Peptide Specificity Assay: 1:1000-1:5000 were incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. No cross-reactivity with peptides containing acetyl-lysine 9 or acetyl-lysine 27 was detected. Chromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from yeast strains containing a Lysine substitution to Alanine at residue 14. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice