H5110-12M9
Histone H3, phosphorylated (Ser28) (Histone H3.1, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ)
Size
£422.00
- SKU:
- H5110-12M9
- Purity:
- Purified by affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Synthetic peptide corresponding to aa23-35 of human Histone H3 conjugated to KLH
- Clone:
- HTA28
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
- Extra Details:
- H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin.Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase H3 has been demonstrated to be phosphorylated by the action of MLTK-a (mixed linage kinase-like mitogen activated protein triple kinase a) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot and Immunoprecipitation. Not recommended for ChIP (Chromatin Immunoprecipitation) assays. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice