Histone H3, phosphorylated (Ser10), acetylated (Ac-K14)

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£1,037.00

SKU:: H5110-12H
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa7-20 of human histone H3 (ARK[pS]TGGAcKAPRKQL-C), KLH-conjugated.
Format:: Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 5mg/ml BSA, 30% glycerol.
Extra Details:: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. Applications: Suitable for use in Western Blot, ChIP and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000-1:5000 ChIP: 1ug/mg of total cell protein (~10e7 cells) Optimal dilutions to be determined by the researcher. Positive Control: Acid-extracted proteins from serum-starved 10T1/2 cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice