H5110-01E
Histone H1
Size
£728.00
- SKU:
- H5110-01E
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Nuclei from myeloid leukemia biopsy cells.
- Clone:
- AE-4
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependent manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30nm chromatin fiber. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence: Speckled staining is observed in the nucleus of normal and malignant cells. Western Blot: 1:100. In blots of rat brain homogenate a duplet is observed at 27-29kD consistent with Histone H1. Immunohistochemistry (Frozen, paraffin): Acetone or PFA fixation of frozen sections is recommended. Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Tonsil Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice