H5109-48D

Histone Deacetylase 3 (HDAC3)

Size

£927.00

SKU:: H5109-48D
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide (NEFYDGDHDNDKESDVEI) corresponding to amino acids 411-428 of human HDAC3. Conjugated to KLH. The immunizing sequence is identical in mouse, rat and chicken.
Clone:: 4H330 (3G6)
Format:: Supplied as a liquid in PBS pH 7.6, 0.07% sodium azide, before the addition of glycerol to 30%.
Extra Details:: Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.5-4ug/ml detects HDAC3 in nuclear extracts from HeLa cells. Immunoprecipitation: 4ug immunoprecipitates HDAC3 from 500ug of HeLa nuclear extract, followed by immunoblot analysis using H5109-48F. Immunoprecipitation/HDAC: 3-15ug immunoprecipitates HDAC3 from 500ug of a HeLa cell nuclear extract. Enzymatic activity was assessed using H5109-62. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.