H5109-48
Histone Deacetylase 3 (HDAC 3)
Size
£581.00
- SKU:
- H5109-48
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the C-terminal sequence of human HDAC 3.
- Format:
- Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Extra Details:
- The histone tail acetylation promotes transcriptional activation. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins and target specific genes. In addition to histones, some HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In another respect, histone deacetylation is involved in silencing of gene transcription (4). Mammalian histone deacetylases can be divided into two classes on the basis of their similarity to various yeast histone deacetylases (5). The first class is represented by its closeness to the yeast Rpd3- like proteins (HDAC 1, 2, 3 and 8), and the second class, which has similarities to yeast Hda1-like proteins (HDAC 4, 5, 6 and 7). Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry: 1:50 (Paraffin) Immunocytochemistry: 1:50 (ABC detection method). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice