H5099-16B
His-Tag (6-Histidine) (HRP)
Size
£704.00
- SKU:
- H5099-16B
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- PAX6 transcription factor linked to histidine tag.
- Clone:
- 3H2201 (AD1.1.10)
- Format:
- Supplied as a liquid in PBS, 0.01% thimerosal, HRP stabilizer. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Insertion of a 6-histidine tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the His-tagged product. Such proteins are purified by immobilized metal ion afftinity chromatography which makes use of its high affinity for transition metal ion. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 ELISA: 1:20-1:1000 Optimal working dilutions to be determined by researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice