H5099-05E
His-Tag (6-Histidine, 6 His, 6-His Epitope Tag, HHHHHH Epitope Tag, HHHHHH Tag, Polyhistidine Tag) (Sepharose)
Size
£704.00
- SKU:
- H5099-05E
- Application:
- IP
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- His-tag-fusion protein.
- Clone:
- 11C113
- Format:
- Supplied as a liquid in PBS, 0.05% azide as 1:1 (v/v) suspension. Labeled with Sepharose. Binding capacity is at least 5-10nmoles of HIS tagged protein per 1ml settled resin.
- Extra Details:
- Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c- terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enables a selective identification and purification of the protein of interest. Poly-His (His x 6) containing proteins can be purified by immobilized metal ion affinity chromatography (IMAC), making use of its high affinity for transition metal ions (1-4). This purification system eliminates the harsh conditions required to elute protein from ligand affinity columns. Numerous recombinant fusion proteins have been engineered with histidine tags to allow detection, isolation and purification of fusion proteins (1-4). Anti- poly may be used to identify the expression of a poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors. Applications: Suitable for use in Immunoprecipitation and for affinity purification of fusion proteins containing His-tags. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: His-tag-fusion protein with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice