H5099-05A-PE

His-Tag (6-Histidine) (PE)

Size

£927.00

SKU:: H5099-05A-PE
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide containing six His residues (6xHis)
Clone:: 4A12E4
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
Extra Details:: The His-tagged expression system is widely used in the production of recombinant protein. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by single-step chromatography. The His-tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest. Applications: Suitable for use in Immunoprecipitation, FLISA and Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: 10ug/application FLISA: 0.1-1ug/ml Western Blot: 1-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.