H5099-05A-FITC
His-Tag (6-Histidine) (FITC)
Size
£927.00
- SKU:
- H5099-05A-FITC
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide containing six His residues (6xHis)
- Clone:
- 4A12E4
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- The His-tagged expression system is widely used in the production of recombinant protein. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by single-step chromatography. The His-tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest. Applications: Suitable for use in Immunoprecipitation, FLISA and Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: 10ug/application FLISA: 0.1-1ug/ml Western Blot: 1-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice