H5099-01A
His-Tag (6-Histidine)
Size
£748.00
- SKU:
- H5099-01A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide containing six histidine residues (6xHis), conjugated to KLH.
- Clone:
- 8A175
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 200ul of sterile ddH2O.
- Extra Details:
- Insertion of a 6-histidine tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the His-tagged product. Such proteins are purified by immobilized metal ion afftinity chromatography which makes use of its high affinity for transition metal ion. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Dot Blot, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence: 1ug/ml Flow Cytometry: 1ug/ml ELISA: 0.05-0.2ug/ml Western Blot: 0.1-0.2ug/ml Immunoprecipitation: 1ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0-Ag14 mouse myeloma cells fused with spleen cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice