Histamine Receptor 4 (H4R)

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£767.00

SKU:: H5090-30F
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 17 aa peptide within the cytoplasmic, C-terminus of human H4R (1) conjugated to KLH
Format:: supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Extra Details:: Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitters. It plays an important role in a multitude of physiological processes in central and peripheral tissues. Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g. sleep/wakefulness, hormonal secretion, cardiovascular control, thermoregulation, food intake, and memory formation). In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells. Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system. Histamine effects are mediated by four pharmacologically distinct receptors, the H1R, H2R , H3R and H4R receptors, which are all members of the G-protein coupled receptor (GPCR) family. Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length. Most recently, a novel orphan G-protein coupled receptor, named H4R (GPRv53, human 390 aa) has been cloned and characterized. It is most closely related to H3R (~37% homology). Unlike H3R, H4R has a distinct tissue distribution and it is localized in the peripheral blood leukocytes, spleen, thymus and colon. Mammalian cells expressing H4R were demonstrated to bind and respond to histamine in a concentration-dependent manner. In functional assays, an H3 receptor agonist, R-(a)-methylhistamine, but also a H3 receptor antagonist, clobenpropit, and a neuroleptic, clozapine, activated H4R-expressing cells. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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