Histamine (Not for Export EU)

Size

£1,138.00

SKU:: H5080-04
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Histamine, conjugated to HSA
Format:: Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O. Further dilutions should be made in PBS, 1% BSA, 0.09% sodium azide.
Extra Details:: Histamine is a neurotransmitter in the central nervous system a well as a mast cell constituent. In addition, histamine is produced by endocrine cells (ECL-cells) in the oxynthic mucosa of the stomach. Applications: Suitable for use in Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:1500-1:2000 (PAP). Immunohistochemistry (Paraffin sections): 1:1500-1:2000 (PAP). Requires HIER. Immunofluorescence (IC): 1:300-1:600 with overnight incubation at 4°C. Optimal dilutions to be determined by the researcher. Recommended Positive Control: Cryostat sections of carbodiimide fixed human skin or freeze-dried paraffin-sections (vapor fixed in diethylpyrocarbonate; DEPC) of rat stomach. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.