H2035-36A

Hexokinase 3, Control Peptide (HXK3, HK3, Hexokinase Type III, HK III)

Size

£416.00

SKU:: H2035-36A
Molecular Weight:: 3
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Extra Details:: Control peptide for H2035-36. Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P. Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate. At least 4 related hexokinase isoforms (HXKI-III; HXK-IV also known as Glucokinase) have been cloned and characterized. Hexokinases (~100kD for HXK1-III; HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins. The N-terminus, containing the mitochondrial target sequence, and the C-terminal has high sequence homology among various isoforms. The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus. HXK3 (chromosome 5) has a more limited distribution that includes white cells and shows a genetically determined polymorphism. It is also expressed in lung and liver. It is 50% identical to HXK1. Source: Synthetic 17aa peptide sequence from mouse HXK3, N-terminal. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice