H2035-36

Hexokinase 3 (HXK3, HK3, Hexokinase Type III, HK III)

Size

£754.00

SKU:: H2035-36
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 17aa at the N-Terminal of mouse HXK3, conjugated to KLH. Species Sequence Homology: rat and human, 94%.
Format:: Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Extra Details:: Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P. Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate. At least 4 related hexokinase isoforms (HXKI-III; HXK-IV also known as Glucokinase) have been cloned and characterized. Hexokinases (~100kD for HXK1-III; HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins. The N-terminus, containing the mitochondrial target sequence, and the C-terminal has high sequence homology among various isoforms. The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus. HXK3 (chromosome 5) has a more limited distribution that includes white cells and shows a genetically determined polymorphism. It is also expressed in lung and liver. It is 50% identical to HXK1. Applications: Suitable for use in ELISA. Other potential application, though not tested is Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml; Coat ELISA plates at 1ug/ml with control peptide. Optimal dilutions to be determined by the researcher. Control Peptide: H2035-36A: Hexokinase 3, Control Peptide (HXK3, HK3, Hexokinase Type III, HK III) Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice