H2034-50A

Heterochromatin Protein 1, alpha (HP1a)

Size

£991.00

SKU:: H2034-50A
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: GST fusion protein corresponding to residues 2-191 from human HP1a.
Clone:: 5H13 (15.19s2)
Format:: Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, pH 7.4, 0.05% sodium azide.
Extra Details:: Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (alpha, beta and gamma) are primarily associated with centromeric heterochromatin; however, HP1b and gamma also localize to euchromatic sites in the genome. HP1 proteins are approximately 25kD in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1). Applications: Suitable for use in Western Blot, Immunohistochemistry and Chromatin Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml in acid-extracted HeLa cell lysates. Immunohistochemistry: Reported to detect HP1a in NIH/3T3 nuclei. Chromatin Immunoprecipitation (ChIP): Reported to immunoprecipitate HP1a from nuclear extracts and formalin-cross-linked chromatin. Optimal dilutions to be determined by the researcher. Recommended Control: HeLa, NIH/3T3, COS cell lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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