HES4 (Hairy Family Helix-loop-helix Transcription Factor)

Size

£843.00

SKU:: H2034-38A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: E. coli-derived recombinant protein corresponding to Val94-Arg221 from human HES-4.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: HES-4 is a 221 aa, 24 kD member of the HES-family of transcriptional repressors. HES-4 contains a species-specific bHLH domain (aa 33-93), a specific orange DNA-binding domain (aa 109-150), a 65 aa Proline-rich SH3-binding domain, and a WRPW Groucho/TLE interacting C- terminal motif. HES-4 is a HES-1 paralog likely to maintain stem cell pluripotency. In the region used as immunogen, the amino acid sequence of human and canine HES-4 are 80% identical. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml with recombinant human HES-4 Immunocytochemistry: 10ug/ml to detect HES-4 in HEK293 cells. C Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.