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H2004-50A

Hepatocyte Cell Adhesion Molecule (HepaCAM)

Size

£819.00

SKU:
H2004-50A
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Sheep
Immunogen:
NS0-derived recombinant protein corresponding to Val34-Tyr242 from human HepaCAM.
Format:
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS
Extra Details:
Hepatocyte Cell Adhesion Molecule (HepaCAM) is a 416 amino acid type I transmembrane glycoprotein that belongs to the immunoglobin superfamily. HepaCAM forms cis-homodimers on the cell surface and may be involved in modulating cell-matrix interactions, likely by promoting cell spreading and motility. Human HepaCAM runs as a 48-72 kD protein depending on glycosylation. It contains a 206 amino acid extracellular domain and a 152 amino acid cytoplasmic domain. Over the extracellular region used as immunogen (aa 34-242), human HepaCAM is 99% and 98% identical to mouse and canine HepaCAM proteins, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml with human brain (motor cortex) tissue and human brain (hippocampus) tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice