H1999-05
Hepatocellular Carcinoma (Hemofiltrate Carcinoma Chemokine, HCC-1)
Size
£594.00
- SKU:
- H1999-05
- Purity:
- Purified by immunoaffinity chromatography. Endotoxin: <0.1ng/ug of protein (<1EU/ug)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human Hepatocellular Carcinoma expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
- Extra Details:
- Human Hemofiltrate CC chemokine-1 (HCC-1) was first isolated from the hemofiltrate of patients with chronic renal failure. HCC-1 has a 46% amino acid sequence identity with MIP-1a/MIP-1b and 29-37% homology with other CC chemokines. Human HCC-1 is an 8.4kD protein containing 72 amino acid residues. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution In conjunction with biotinylated anti-human Hepatocellular Carcinoma (H1999-05A) as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human Hepatocellular Carcinoma. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Hepatocellular Carcinoma is 1.5-3ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry (FFPE): 1.5ug/ml with an overnight incubation at 4ºC. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice