H1920-22P
Hepatitis C Virus NS3 (HCV)
Size
£646.00
- SKU:
- H1920-22P
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified Recombinant Chimeric HCV Polyprotein (555aa residues)
- Clone:
- 3H156
- Format:
- Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile ddH2O.
- Extra Details:
- Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-1ug/ml will allow visualization of 100ng/lane of both Recombinant Chimeric HCV Polyprotein and Recombinant NS-3 Protein. No reaction can be seen with Synthetic NS-3. Testing is under reducing and non-reducing conditions. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0-AG14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice