H1920-14A
Hepatitis C Virus Core Protein (HCV)
Size
£288.00
- SKU:
- H1920-14A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptides derived from HCV core protein
- Clone:
- 3H149 (A1/3D1)
- Format:
- Supplied as a liquid in PBS, pH 7.2.
- Extra Details:
- Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of Core Protein (Capsid Protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6kB HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B. Applications: Suitable for use in ELISA. Not suitable for Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Indirect): The testing plates were coated with HCV non-structural protein (NS), core protein (C) and the mixture of NS and C respectively. The neat culture supernatant of hybridoma showed a strong reaction with HCV capsid protein (OD492 value >2) and with mixture of NS and C (OD492 value >1.5). Showed no reaction with HCV NS (OD492 value <0.03). Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0-AG14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice