H1905-13

Hepatitis Bx Antigen (HBxAg)

Size

£709.00

SKU:: H1905-13
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: HBxAg-Protein A Fusion protein.
Clone:: 3H2188 (3F6-G10)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:500 Immunohistochemistry (Frozen and paraffin): 1:100; Paraffin sections do not require protein digestion pre-treatment or antigen retrieval. Optimal dilutions to be determined by the researcher. Recommended Postive Control Tissue: Liver carcinoma/Hepatitis B infected liver Recommoned Secondary Antibodies: I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt x Mo I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb x Mo Recommended Negative Control: I1904-83P1: IgG2a Murine Negative Control Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of a mouse myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.