H1890-93B
Heparan-Sulfate Proteoglycan
Size
£390.00
- SKU:
- H1890-93B
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human small basement membrane heparan sulfate proteoglycan (HS-PG)
- Clone:
- 2H7/G11
- Format:
- Supplied as a lyophilized powder in 50mM TBS, pH 7.2. Reconstitute with 10/100ul sterile ddH2O.
- Extra Details:
- The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200kD (aorta) and 30-160kD (kidney) and a core protein size of 24kD or 22kD, respectively, was localized by immunohisto-hemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1ug/ml Western Blot: 1ug/ml Immunohistochemistry: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice