H1890-90B

Heparan Sulfate Proteoglycan (Perlecan, HSPG)

Size

£1,077.00

SKU:: H1890-90B
Purity:: Purified by Protein A/G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bovine corneal endothelial cells. Epitope: domain V.
Clone:: 6F166 (A74)
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450kD and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability charateristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling (2). Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation. Can also be used in affinity chromatography, in conjunction with H1890-90A to separate recombinant domain I from full length perlecan. Other applications not tested. Recommended Dilutions: ELISA: 1:2000 Western Blot: 1:100 Immunohistochemistry: 1:100. Frozen PLP-fixed sections of bovine and human tissues Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.