Heme Oxygenase 3 (HO-3) (Not for Export EU)

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£704.00

SKU:: H1847-95
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: An 11-aa peptide sequence within the N-terminus of rat HO-3 (1) was synthesized, coupled to KLH
Format:: Supplied as a liquid, neat serum, 40% glycerol.
Extra Details:: Heme oxygenase is the rate-limiting microsomal enzyme in the heme degradative pathway. Heme oxygenase catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to form equimolar biliverdin, carbon monoxide, a putative neurotransmitter, and iron. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator; biliverdin and its product bilirubin are potent antioxidants; "free' iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferrin receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'-UTRs of the mRNAs. To date, 3 forms of heme oxygenases (HO1-3) have been identified. A third enzyme, HO-3 (rat 290 aa ~ 33kD) has been cloned and characterized from rat brain. The HO-3 mRNA is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. The predicted amino acid structure of HO-3 differs from both HO-1 and HO-2 but is closely related to HO-2 (~ 90% similarity). HO-3 sequence has two HRM known to be involved in heme binding. It is a poor heme catalyst. Therefore, HO-3 has a potential regulatory role in heme-dependent cellular processes. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for aff. pure IgG using ECL. HO-2 ~32-34kD. ELISA: ~1:100,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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