H1847-53X
Hemagglutinin Tag (YPYDVPDYA) (HA Tag)
Size
£800.00
- SKU:
- H1847-53X
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide containing the sequence YPYDVPDYA derived from the influenza virus hemaglutinin (HA) protein.
- Clone:
- 3G165
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- The HA tag sequence is derived from influenza hemagglutinin and is typically used to facilitate the detection of proteins. Tags such as HA used in conjunction with proteins can aid in purification, identification, and functional analysis of the tagged protein.Through the use of standard molecular biology techniques, a cDNA, which encodes the protein of interest can be cloned, in- frame, into an expression vector containing the tag sequence. The resultant tagged protein is usually referred to as an epitope-tagged protein or a fusion protein. Currently, a variety of expression vectors containing tags are commercially available. Depending on the expression vector selected, these fusion proteins may be expressed in a variety of organisms including bacteria, yeast, insect cells, and mammalian cells. Detection of a tagged protein is facilitated by the use of an antibody directed against the particular tag. This alleviates the need to generate a specific antibody against the protein itself. Therefore, newly identified proteins can be expressed and studied without having to wait for a specific antibody to be generated. Some of the more commonly used fusion tags include: c-myc, FLAG, GFP, GST, HA, His, and MBP. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Immunofluorescence (IC): 2ug/ml Optimal dilutions to be determined by the researcher. Positive control: HA-tagged JNK-1 transfected cell lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice