Hemagglutinin Tag (YPYDVPDYA) (HA Tag)

Size

£735.00

SKU:: H1847-51G
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH conjugated epitope tag peptide YPYDVPDYA (aa114-122) from hemagglutinin influenza.
Format:: Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH7.2, 0.01% sodium azide.
Extra Details:: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000 Western Blot: 1:2000-1:10,000 Immunohistochemistry (paraffin): 1:500-1:2000. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice